Twins and High-order Multiple Gestations

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Last updated: June 26, 2026Bookmark

Multiple gestations refer to a pregnancy with more than one fetus.

A twin pregnancy is when two fetuses develop in the uterus simultaneously.

Multiple gestations are associated with a higher rate of almost every potential complication of pregnancy, excluding post-term delivery and macrosomia. The best outcome in multiple gestations is seen in diamniotic, dichorionic twinssince each fetus has their own nutrient supply.

70% of twins are dizygotic, and 30% are monozygotic.

36% of twin pregnancies and 77% of higher order pregnancies occur following infertility treatment, e.g., in vitro fertilisation or clomiphene.

Definition of terms

TermsDescription
MonozygoticIdentical twins arising from a single zygote
DizygoticNon-identical twins from two different zygotes
MonoamnioticA single amniotic sac
DiamnioticTwo separate amniotic sacs
MonochorionicShares a single placenta
DichorionicTwo separate placentas
SuperfecundationFertilization of two separate ova released in the same menstrual cycle resulting in dizygotic twins
Heteroplacental superfecundationDizygotic (dichorionic diamniotic) twins with two different fathers
SuperfetationFertilization and implantation of a second embryo during an ongoing pregnancy resulting in two fetuses of different gestational age

Summary of monozygotic twin gestation

Dichorionic-diamnioticMonochorionic-diamnioticMonochorionic-monoamniotic
Frequency25%60 – 70%1 – 2%
Time of separation≤ 72 hours4 – 8 days9 – 12 days
Stage of separationMorula stageBlastocyst stageEmbryonic stage
StucturesSeparate chorion and amniotic sacShared chorion and separate amniotic sacShared chorion and shared amniotic sac
Ultrasound findingsLambda sign (intertwin membrane + twin peak)T-sign (intertwin membrane)No intertwin membrane
Associated complicationsFewer rates of complicationsTwin-to-twin transfusion syndrome (TTTS)Twin-to-twin transfusion syndrome (TTTS), Cord accidents
  • Risk factors for multiple gestation
    • Artificial reproductive technology
    • Clomiphene
    • Human menopausal gonadotropin
    • African heritage
    • Multiparity
    • Family history of multiple gestation
    • Obesity
    • Tall mother (the taller the mother the higher the risk of twinning)
    • Geography (South west nigeria has a 1 in 20 risk of twinning)
  • Signs and symptoms
    • Increased incidence of hyperemesis gravidarum
    • Larger than expected symphysiofundal height
    • Quantitafive hCG and AFP high relative to date
  • Investigations
    • Complete blood count to monitor for anaemia at first visit, 20 weeks and 28 weeks
    • Ultrasound for diagnosis at first visit
      • Lamda sign or twin peak sign – triangular appearance of membranes (dichorionic diamniotic)
      • T-sign – membranes abruplty meet the chorion (monochorionic diamniotic)
      • No membrane separating the twins in monochorionic monoamniotic twins
  • Treatment
    • Nutritional supplementation including folate and iron.
    • 10-14 weeks
      • Ultrasound to determine chorionicity or amnionicity
    • 18-22 weeks
      • Anatomy ultrasound
    • Serial ultrasounds during the 2nd and 3rd trimester (frequency depends on chorionicity)
      • 2 weekly from 16 weeks for monochorionic twins (have a high risk of twin-to-twin transfusion)
      • 4 weekly from 20 weeks for dichorionic twins
      • 4 – 6 weekly for dizygotic twins
      • Cervical length measurements to predict preterm labour
    • 28 weeks
      • Antenatals steroids for monochorionic monoamniotic twins
    • Delivery
      • Prescribe antenatal steroids
      • Elective caesarean delivery for:
        • Monoamniotic twins (due to risk of cord prolapse in the second twin)
        • Diamniotic twins when the presenting twin is not in cephalic presentation (possibility of locked twins)
        • May be required for delivery of the second twin after successful birth of the first twin
      • Vaginal delivery for:
        • Diamniotic twins when the first twin is in cephalic presentation

Timing of delivery

TimingTwins
32 – 34 weeksUncomplicated monochorionic monoamniotic twins
36 – 37 weeksUncomplicated monochorionic diamniotic twins
37 – 38 weeksUncomplicated dichorionic diamniotic twins
Before 35 + 6 weeksFor triplets. 75% of triplets deliver before 35 weeks
Induction at any timeSignificant fetal growth retriction

Complications of Multiple Gestation

ClassificationComplication
Maternal complicationsHyperemesis gravidarum, iron deficiency anaemia, intrahepatic cholestasis of pregnancy, venous thromboembolism
Fetal complicationsPrematurity, cerebral palsy, discordant growth, congenital anomalies, stillbirth, fetal growth restriction, twin-twin transfusion syndrome, twin anaemia polycythaemia sequence
Obstetric complicationsPre-eclampsia and eclampsia, uterine atony and post-partum hemorrhage, postpartum depression, instrumental delivery or caesarean delivery
Complications affecting the 2nd twinLow birth weight, malpresentation, cord prolapse, abruptio placentae
Complications with one intrauterine fetal demise (IUFD)Disseminated intravascular coagulopathy, chorioamnionitis
Reference Intervals ›
Biochemistry
ACTHP: <80 ng/L
ALTP: 5–35 U/L
AlbuminP: 35–50 g/L
AldosteroneP: 100–500 pmol/L
Alk. phosphataseP: 30–130 U/L
α-AmylaseP: 0–180 IU/dL
α-FetoproteinS: <10 kU/L
Angiotensin IIP: 5–35 pmol/L
ADHP: 0.9–4.6 pmol/L
ASTP: 5–35 U/L
BicarbonateP: 24–30 mmol/L
BilirubinP: 3–17 μmol/L
BNPP: <50 ng/L
CRPP: <10 mg/L
CalcitoninP: <0.1 mcg/L
Calcium (ionized)P: 1.0–1.25 mmol/L
Calcium (total)P: 2.12–2.60 mmol/L
ChlorideP: 95–105 mmol/L
CholesterolP: <5.0 mmol/L
VLDLP: 0.128–0.645 mmol/L
LDLP: <2.0 mmol/L
HDLP: 0.9–1.93 mmol/L
Cortisol AMP: 450–700 nmol/L
Cortisol MidnightP: 80–280 nmol/L
CK ♂P: 25–195 U/L
CK ♀P: 25–170 U/L
CreatinineP: 70–100 μmol/L
FerritinP: 12–200 mcg/L
FolateS: 2.1 mcg/L
FSHP: 2–8 U/L ♂; >25 menopause
GGT ♂P: 11–51 U/L
GGT ♀P: 7–33 U/L
Glucose (fasting)P: 3.5–5.5 mmol/L
Growth hormoneP: <20 mu/L
HbA1C (DCCT)B: 4–6%
HbA1C (IFCC)B: 20–42 mmol/mol
Iron ♂S: 14–31 μmol/L
Iron ♀S: 11–30 μmol/L
Lactate (venous)P: 0.6–2.4 mmol/L
Lactate (arterial)P: 0.6–1.8 mmol/L
LDHP: 70–250 U/L
LHP: 3–16 U/L
MagnesiumP: 0.75–1.05 mmol/L
OsmolalityP: 278–305 mosmol/kg
PTHP: 0.8–8.5 pmol/L
PotassiumP: 3.5–5.3 mmol/L
Prolactin ♂P: <450 U/L
Prolactin ♀P: <600 U/L
PSAP: 0–4 mcg/mL
Protein (total)P: 60–80 g/L
Red cell folateB: 0.36–1.44 μmol/L
Renin (erect)P: 2.8–4.5 pmol/mL/h
Renin (recumbent)P: 1.1–2.7 pmol/mL/h
SodiumP: 135–145 mmol/L
TBGP: 7–17 mg/L
TSHP: 0.5–4.2 mU/L
T4P: 70–140 nmol/L
Free T4P: 9–22 pmol/L
TIBCS: 54–75 μmol/L
TriglyceridesP: 0.50–2.3 mmol/L
T3P: 1.2–3.0 nmol/L
Troponin TP: <0.1 mcg/L
Urate ♂P: 210–480 μmol/L
Urate ♀P: 150–390 μmol/L
UreaP: 2.5–6.7 mmol/L
Vitamin B12S: 0.13–0.68 nmol/L
Vitamin DS: 50 nmol/L
Arterial Blood Gases
pH7.35–7.45
PaCO₂4.7–6.0 kPa
PaO₂>10.6 kPa
Base excess±2 mmol/L
Urine
Cortisol (free)<280 nmol/24h
Hydroxyindole acetic acid16–73 μmol/24h
Hydroxymethylmandelic acid16–48 μmol/24h
Metanephrines0.03–0.69 μmol/mmol cr.
Osmolality350–1000 mosmol/kg
17-Oxogenic steroids ♂28–30 μmol/24h
17-Oxogenic steroids ♀21–66 μmol/24h
17-Oxosteroids ♂17–76 μmol/24h
17-Oxosteroids ♀14–59 μmol/24h
Phosphate (inorganic)15–50 mmol/24h
Potassium14–120 mmol/24h
Protein<150 mg/24h
Protein/creatinine ratio<3 mg/mmol
Sodium100–250 mmol/24h
Haematology
WCC4.0–11.0 ×10⁹/L
RBC ♂4.5–6.5 ×10¹²/L
RBC ♀3.9–5.6 ×10¹²/L
Hb ♂130–180 g/L
Hb ♀115–160 g/L
PCV ♂0.4–0.54 L/L
PCV ♀0.37–0.47 L/L
MCV76–96 fL
MCH27–32 pg
MCHC300–360 g/L
RDW11.6–14.6%
Neutrophils2.0–7.5 ×10⁹/L (40–75%)
Lymphocytes1.0–4.5 ×10⁹/L (20–45%)
Eosinophils0.04–0.44 ×10⁹/L (1–6%)
Basophils0–0.10 ×10⁹/L (0–1%)
Monocytes0.2–0.8 ×10⁹/L (2–10%)
Platelets150–400 ×10⁹/L
Reticulocytes0.8–2.0% / 25–100 ×10⁹/L
Prothrombin time10–14 s
APTT35–45 s
Paediatric
Pulse Rate (bpm)
Neonate140–160
Infant <1yr120–140
1–5 years110–130
5–12 years80–120
>12 years70–100
Respiratory Rate (tachypnoea)
0–2 months≥60/min
2–12 months≥50/min
1–5 years≥40/min
>5 years≥30/min
Blood Pressure (mmHg)
Term65/45
1 year75/50
4 years85/60
8 years95/65
10 years100/70
Weight Formulas
3–12 months(a + 9)/2 kg
1–6 years2a + 8 kg
>6 years(7a − 5)/2 kg
Haemoglobin (g/dL)
Term newborn13–20
1 month11–18
2 months10–15
1–2 years10–13
>2 years11–14
MUAC (6 months–5 years)
Obese>17.5 cm
Normal13.5–17.4 cm
At risk12.5–13.4 cm
Moderate malnutrition11.5–12.4 cm
Severe malnutrition<11.5 cm
Developmental Milestones
Social smile1.5 months
Head control4 months
Sits unsupported7 months
Crawls10 months
Stands unsupported10–12 months
Walks12–13 months
Talks18 months
CSF WBC (/mm³)
Term newborn0–25
>2 weeks0–5
Calculator ›

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