Lymphadenopathy

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Last updated: May 8, 2026Bookmark

Lymphadenopathy is defined as a lymph node that is greater than 1 cm in diameter. Most cases of lymphadenopathy are related to infections: these resolve independently or after antibiotic treatment.

Lymph node sites

Lymph node siteArea drained
CervicalHead and neck
Submental and submandibularBuccal mucosa, cheek, and nose
Right supraclavicularThorax
Left supraclavicularAbdomen
AxillaryIpsilateral arm, breast, neck, and thorax
InguinalIpsilateral leg, buttock, and lower half of abdominal wall

Characteristics of lymph nodes on palpation

FindingDescription
Firm, rubbery, or hardThis is concerning for malignancy. The texture is caused by infiltration of malignant cells. A hard lymph node feels like a stone – like the forehead (metastatic cancer); a firm or rubbery lymph node feels firmer than surrounding fat tissue – like the tip of the nose (lymphoma); a soft consistency is more likely due to infection or inflammation
FluctuantFeels like an abscess. May contain fluid and be compressible. Commonly associated with infections
MobileCan be freely moved within the surrounding tissue
FixedStuck to the surrounding tissue and is immobile
ShottyMany tiny, scattered lymph nodes in the same area. Occurs in cervical lymph nodes after viral infection in children
MattedLymph nodes that feel connected or form a continuous mass. Associated with malignancy or chronic inflammation, e.g., tuberculosis
TenderAssociated with inflammatory or infectious causes. It can also occur in malignancy due to necrosis or hemorrhage into the lymph node

Causes of lymphadenopathy

CauseDescription
Viral infectionThe most common cause in children. URTI causes cervical lymphadenopathy. Should resolve in 4 – 7 days without antimicrobial treatment. Up to 18% of HIV-affected patients in Africa have lymphadenopathy on presentation
Bacterial infectionReactive lymphadenopathy in lymph nodes that drain the area of infection. May result in abscess formation within the lymph node. Abscesses < 1.5 cm are treated with antibiotics, while those > 1.5 cm should be drained.
Tuberculosis lymphadenitis (scrofula)The most common extrapulmonary manifestation of TB. Typically in the supraclavicular lymph nodes, but it can also affect other areas. Progresses to ‘cold abscesses’ and then to draining sinuses. Aspiration shows caseous or purulent material. Diagnosed by FNA with PCR, though Chest X-ray or PPD may be suggestive.
Atypical mycobacteria (MOTT)A cause of lymphadenopathy in children, especially of the facial or cervical regions. Usually involves submental nodes. Similar to tuberculous lymphadenopathy. Treated with clarithromycin and rifampin for 6-9 months
Malignancy12% of biopsied lymph nodes are malignant. Lymphoma is the most likely cause (70%). Other rare causes are head and neck cancer, metastasis, and Kaposi’s sarcoma.
  • Patient History
    • Location
    • Duration
    • Change in size
    • Treatments performed and response
    • Presence of B-symptoms
      • Fever
      • Night sweats
      • Easy bruising
      • Fatigue
      • Weight loss
    • Travel, animal, or insect exposure
    • Medical history, including infectious diseases, e.g,. tuberculosis and HIV
  • Physical examination
    • Location:
      • Examine all lymph node basins – submental, submandibular, parotid, anterior cervical, posterior cervical, supraclavicular, axillary, and inguinal regions
    • Size
    • Palpation and description of findings
    • Other physical exam findings:
      • Skin changes
      • Bruising
      • Rashes and other skin lesions
      • Neck range of motion
      • Hepatosplenomegaly
  • Investigations
    • Complete blood count to identify infectious etiologies and hematologic malignancies
    • Liver function tests, depending on the history
    • HIV, EBV, CMV, Hepatitis ,B and Herpes Serology
    • Chest radiograph to identify mediastinal lymphadenopathy, which is associated with lymphoma, and lung pathology consistent with tuberculosis
    • Ultrasound for information about the size, shape, and number of lymph nodes and their morphologies and vascular patterns
    • Excisional biopsy is the gold standard test for tissue diagnosis since it allows the tissue architecture to be visualized when the whole node is removed
    • Fine needle aspirate (FNA) is not recommended for paediatric lymphadenopathy due to false-negative results
  • Indications for biopsy
    • Suspicion of malignancy
    • Persistent lymphadenopathy (after 4 – 6 weeks)
    • Size > 2 cm
    • Increase in size over 2 – 3 weeks
    • Multiple nodes with concerning features on ultrasound or CT
Reference Intervals
Biochemistry
ACTHP: <80 ng/L
ALTP: 5–35 U/L
AlbuminP: 35–50 g/L
AldosteroneP: 100–500 pmol/L
Alk. phosphataseP: 30–130 U/L
α-AmylaseP: 0–180 IU/dL
α-FetoproteinS: <10 kU/L
Angiotensin IIP: 5–35 pmol/L
ADHP: 0.9–4.6 pmol/L
ASTP: 5–35 U/L
BicarbonateP: 24–30 mmol/L
BilirubinP: 3–17 μmol/L
BNPP: <50 ng/L
CRPP: <10 mg/L
CalcitoninP: <0.1 mcg/L
Calcium (ionized)P: 1.0–1.25 mmol/L
Calcium (total)P: 2.12–2.60 mmol/L
ChlorideP: 95–105 mmol/L
CholesterolP: <5.0 mmol/L
VLDLP: 0.128–0.645 mmol/L
LDLP: <2.0 mmol/L
HDLP: 0.9–1.93 mmol/L
Cortisol AMP: 450–700 nmol/L
Cortisol MidnightP: 80–280 nmol/L
CK ♂P: 25–195 U/L
CK ♀P: 25–170 U/L
CreatinineP: 70–100 μmol/L
FerritinP: 12–200 mcg/L
FolateS: 2.1 mcg/L
FSHP: 2–8 U/L ♂; >25 menopause
GGT ♂P: 11–51 U/L
GGT ♀P: 7–33 U/L
Glucose (fasting)P: 3.5–5.5 mmol/L
Growth hormoneP: <20 mu/L
HbA1C (DCCT)B: 4–6%
HbA1C (IFCC)B: 20–42 mmol/mol
Iron ♂S: 14–31 μmol/L
Iron ♀S: 11–30 μmol/L
Lactate (venous)P: 0.6–2.4 mmol/L
Lactate (arterial)P: 0.6–1.8 mmol/L
LDHP: 70–250 U/L
LHP: 3–16 U/L
MagnesiumP: 0.75–1.05 mmol/L
OsmolalityP: 278–305 mosmol/kg
PTHP: 0.8–8.5 pmol/L
PotassiumP: 3.5–5.3 mmol/L
Prolactin ♂P: <450 U/L
Prolactin ♀P: <600 U/L
PSAP: 0–4 mcg/mL
Protein (total)P: 60–80 g/L
Red cell folateB: 0.36–1.44 μmol/L
Renin (erect)P: 2.8–4.5 pmol/mL/h
Renin (recumbent)P: 1.1–2.7 pmol/mL/h
SodiumP: 135–145 mmol/L
TBGP: 7–17 mg/L
TSHP: 0.5–4.2 mU/L
T4P: 70–140 nmol/L
Free T4P: 9–22 pmol/L
TIBCS: 54–75 μmol/L
TriglyceridesP: 0.50–2.3 mmol/L
T3P: 1.2–3.0 nmol/L
Troponin TP: <0.1 mcg/L
Urate ♂P: 210–480 μmol/L
Urate ♀P: 150–390 μmol/L
UreaP: 2.5–6.7 mmol/L
Vitamin B12S: 0.13–0.68 nmol/L
Vitamin DS: 50 nmol/L
Arterial Blood Gases
pH7.35–7.45
PaCO₂4.7–6.0 kPa
PaO₂>10.6 kPa
Base excess±2 mmol/L
Urine
Cortisol (free)<280 nmol/24h
Hydroxyindole acetic acid16–73 μmol/24h
Hydroxymethylmandelic acid16–48 μmol/24h
Metanephrines0.03–0.69 μmol/mmol cr.
Osmolality350–1000 mosmol/kg
17-Oxogenic steroids ♂28–30 μmol/24h
17-Oxogenic steroids ♀21–66 μmol/24h
17-Oxosteroids ♂17–76 μmol/24h
17-Oxosteroids ♀14–59 μmol/24h
Phosphate (inorganic)15–50 mmol/24h
Potassium14–120 mmol/24h
Protein<150 mg/24h
Protein/creatinine ratio<3 mg/mmol
Sodium100–250 mmol/24h
Haematology
WCC4.0–11.0 ×10⁹/L
RBC ♂4.5–6.5 ×10¹²/L
RBC ♀3.9–5.6 ×10¹²/L
Hb ♂130–180 g/L
Hb ♀115–160 g/L
PCV ♂0.4–0.54 L/L
PCV ♀0.37–0.47 L/L
MCV76–96 fL
MCH27–32 pg
MCHC300–360 g/L
RDW11.6–14.6%
Neutrophils2.0–7.5 ×10⁹/L (40–75%)
Lymphocytes1.0–4.5 ×10⁹/L (20–45%)
Eosinophils0.04–0.44 ×10⁹/L (1–6%)
Basophils0–0.10 ×10⁹/L (0–1%)
Monocytes0.2–0.8 ×10⁹/L (2–10%)
Platelets150–400 ×10⁹/L
Reticulocytes0.8–2.0% / 25–100 ×10⁹/L
Prothrombin time10–14 s
APTT35–45 s
Paediatric
Pulse Rate (bpm)
Neonate140–160
Infant <1yr120–140
1–5 years110–130
5–12 years80–120
>12 years70–100
Respiratory Rate (tachypnoea)
0–2 months≥60/min
2–12 months≥50/min
1–5 years≥40/min
>5 years≥30/min
Blood Pressure (mmHg)
Term65/45
1 year75/50
4 years85/60
8 years95/65
10 years100/70
Weight Formulas
3–12 months(a + 9)/2 kg
1–6 years2a + 8 kg
>6 years(7a − 5)/2 kg
Haemoglobin (g/dL)
Term newborn13–20
1 month11–18
2 months10–15
1–2 years10–13
>2 years11–14
MUAC (6 months–5 years)
Obese>17.5 cm
Normal13.5–17.4 cm
At risk12.5–13.4 cm
Moderate malnutrition11.5–12.4 cm
Severe malnutrition<11.5 cm
Developmental Milestones
Social smile1.5 months
Head control4 months
Sits unsupported7 months
Crawls10 months
Stands unsupported10–12 months
Walks12–13 months
Talks18 months
CSF WBC (/mm³)
Term newborn0–25
>2 weeks0–5
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